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optimum silicon carbide method of gene transfer

Biomass and lipid induction strategies in microalgae for biofuel …

2019/10/21· Jeon et al. used silica nanoparticles and methyl-functionalized silica (SiO 2 –CH 3) nanoparticles in a C. vulgaris culture. They used Blue-Green medium (BG-11) and grew microalgae that used solely CO 2 as a carbon source; they were able to observe that the NPs increased the gas–liquid mass transfer rate in this CO 2 /medium culture system and improved both growth and lipid …

Agrobacterium tumefaciens-mediated transformation of tobacco …

The Agrobacterium tumefaciens-mediated transformation method is commonly used to create transgenic plants because it has several merits compared with direct gene transfer methods such as particle boardment, electroporation, and silicon carbide fibers

Biolistics - an overview | ScienceDirect Topics

Biolistics. Biolistics, short for “biological ballistics” and also known as particle-mediated gene transfer, is the method of directly shooting DNA fragments into cells using a device called a gene gun. To use a gene gun, a scientist first mixes a DNA construct with particles of a heavy metal, usually tungsten or gold.

Optimization of Agrobacterium-mediated transformation conditions in …

2007/9/29· Many methods have been used to introduce cloned DNA sequences into wheat cells, such as particle boardment [4, 32], electroporation [], DNA uptake by germinating pollen [], polyethylene glycol-mediated gene transfer [], puncture technique of microbeam].

Nonlinear optical imaging of defects in cubic silicon carbide …

2014/6/11· The widespread appliion of silicon carbide power devices is however limited by the presence of structural defects in silicon carbide epilayers. Our experiment demonstrates that optical second harmonic generation imaging represents a viable solution for characterizing structural defects such as stacking faults, disloions and double positioning boundaries in cubic silicon carbide layers.

Silicon Carbide Whisker-Mediated Eryogenic Callus …

2008/7/29· A silicon carbide whisker-mediated gene transfer system with recovery of fertile and stable transformants was developed for cotton (Gossypium hirsutum L.) Silicon Carbide Whisker-Mediated Eryogenic Callus Transformation of Cotton (Gossypium hirsutum L.) and Regeneration of Salt Tolerant Plants | SpringerLink

Silicon carbide whisker-mediated eryogenic callus …

2008/7/29· Europe PMC is an archive of life sciences journal literature. A silicon carbide whisker-mediated gene transfer system with recovery of fertile and stable transformants was developed for cotton (Gossypium hirsutum L.) cv. Coker-312.

High-Efficiency Transformation of Chlamydomonas reinhardtii by Electroporation …

1998/4/1· ethanol. Isolated DNA (5 μg) was digested with SacI fractionated on an agarose gel, and then analyzed by standard Southern blot hybridization procedures (Sarook et al. 1989).RESULTS Enhancement of plating efficiency using the starch eedding method: Cell wall-less strains of C. reinhardtii have considerably lower plating efficiencies than those that synthesize a wall (Harris 1989).

Silicon carbide whisker-mediated eryogenic callus …

A silicon carbide whisker-mediated gene transfer system with recovery of fertile and stable transformants was developed for cotton (Gossypium hirsutum L.) cv. Coker-312.

Improving the nutritional value of Golden Rice through increased …

2005/4/1· For Golden Rice 2, a Glu::SSUcrtI::nos cassette was transferred into a Bin19-based binary vector containing the E. coli phospho-mannose isomerase (PMI) marker gene 28 under the control of …

Vector less gene transfer - SlideShare

2015/11/13· Vector less gene transfer 1. SAAKRE MANJESH 2014-11-105 VECTOR LESS GENE TRANSFER/DIRECT GENE TRANSFER METHODS 2. Join the community of over 1 million readers Sign Up to Scribd to continue downloading Sign up for a Scribd 60 day

Comparison of transformation efficiency of five Agrobacterium …

advantages as compared to direct gene transfer methods such as particle boardment, electroporation, and silicon carbide fibers. The advantages are (1) stable gene expression because of the insertion of the foreign gene into the host transgene; and (3) large

Biolistics - an overview | ScienceDirect Topics

Biolistics. Biolistics, short for “biological ballistics” and also known as particle-mediated gene transfer, is the method of directly shooting DNA fragments into cells using a device called a gene gun. To use a gene gun, a scientist first mixes a DNA construct with particles of a heavy metal, usually tungsten or gold.

Agrobacterium-mediated transformation of maize | Nature …

2007/6/21· Other direct gene transfer methods, which did not require the prior culture of protoplasts, were then tried 2,3,4,5, and microprojectile boardment 6 of cells in suspension cultures or immature

Agrobacterium tumefaciens-mediated transformation of tobacco …

The Agrobacterium tumefaciens-mediated transformation method is commonly used to create transgenic plants because it has several merits compared with direct gene transfer methods such as particle boardment, electroporation, and silicon carbide fibers

UNIVERSITI PUTRA MALAYSIA OPTIMIZATION OF PARAMETERS INVOLVED IN THE TRANSFORMATION OF OIL PALM USING THE BIOLISTIC METHOD …

"By command of my Lord: Of knowledge it is only a little that is communied (thought) to you (0 men!)"(Surah AI-Isra'' : 85) "It is He Who produces gardens, with trellises and without, and date-palms, and crops of diverse flavour, and the olive and the pomegranate,

Viral RNA Extraction: Norgen vs. Zymo Comparison

Norgen’s silicon carbide-based Saliva/Swab RNA Purifiion Kit (. 69100) provides a highly sensitive, simple and reliable method for RNA extraction from virus-containing samples. This was demonstrated by successful de-tection of as few as 10 copies in a

How to Transform Microalgae - Bitesize Bio

2019/8/8· In this method, macro or microparticles (e.g. glass beads or silicon carbide whiskers), the gene(s) of interest to be delivered, denatured fish sperm DNA which binds to nucleases in the cell and prevents degradation of the gene(s) of interest, as well as

Viral RNA Extraction: Norgen vs. Zymo Comparison

Norgen’s silicon carbide-based Saliva/Swab RNA Purifiion Kit (. 69100) provides a highly sensitive, simple and reliable method for RNA extraction from virus-containing samples. This was demonstrated by successful de-tection of as few as 10 copies in a

17. Transgenic Breeding - PlantBreeding

2013/8/9· Methods of Plant Transformation Direct Transformation There are a variety of direct DNA delivery methods to recover transgenic plants including, but not limited to, silicon-carbide whisker-mediated transformation, microinjection, protoplast transformation using

Optimization of Agrobacterium-mediated transformation conditions in …

2007/9/29· Many methods have been used to introduce cloned DNA sequences into wheat cells, such as particle boardment [4, 32], electroporation [], DNA uptake by germinating pollen [], polyethylene glycol-mediated gene transfer [], puncture technique of microbeam].

Nanoluciferase-Based Method for Detecting Gene Expression in …

2019/12/1· Unless indied otherwise, the optimal gain across the plate was determined from the sample with the highest luminescent signal. For a blank, 1× lysis buffer vortexed with silicon carbide beads and Nano-Glo reagent were used.

The Future of Neuroscience: Flexible and Wireless Implantable …

When using silicon as a probe substrate, there must be some method of shaping a wafer into a thin probe shaft. Wafer dicing often produces fragile chips, typically with defects; [ 185 ] despite this, it is employed as a simple and repeatable approach to achieve the probe shape.

Uptake, Transloion, and Consequences of Nanomaterials on …

2021/3/16· Uptake, Transloion, and Consequences of Nanomaterials on Plant Growth and Stress Adaptation. Shahid Ali,1 Asif Mehmood,2 and Naeem Khan 3. 1College of Life Sciences, Northeast Forestry University, Harbin 150040, China. 2Institute of Biological Sciences, Sarhad University of Science and Information Technology, Peshawar 25000, Pakistan.

Method of protein purifiion - Norgen Biotek Corporation

Purifiion methods are provided for proteins and peptides, employing silicon carbide to bind the proteins or peptides. The methods may also be used to recover and purify recoinantly expressed proteins sequestered in inclusion bodies. The method for purifying a

Electroporation-mediated and silicon carbide fiber-mediated …

1991/1/1· The results of DNA delivery experiments in Agrostis alba by electroporation into eryogenic suspension culture-derived protoplasts and by the silicon carbide fiber-mediated method into intact suspension cells are described, using the two plasmid constructs, pFF19G and wheat dwarf virus (WDV)-derived pWI-GUS.

Whisker-mediated transformation of peanut with chitinase gene …

Silicon carbide whiskers on the other hand are extremely well-suited means of direct gene delivery, for being very simple and not requiring much technical equipment and skills (Kaeppler et al. 1990). These whiskers are basically 10-80 mm long and 0.6 mm widemicrofibers ( Coffee and Dunwell 1994 ).

Transformation of plant cells - Zeneca Limited

Silicon carbide fibres (30 μm×0.5 μm) were sterilised under ultraviolet light, then suspended in sterile water to give a 5% solution. The DNA construct used was a plasmid designated pAI 1 gusN, containing the Adh 1 promoter and the β-glucuronidase (GUS) reporter gene.

Nonlinear optical imaging of defects in cubic silicon carbide …

2014/6/11· The widespread appliion of silicon carbide power devices is however limited by the presence of structural defects in silicon carbide epilayers. Our experiment demonstrates that optical second harmonic generation imaging represents a viable solution for characterizing structural defects such as stacking faults, disloions and double positioning boundaries in cubic silicon carbide layers.

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